Infertility Investigations:
Initial Assessment (GP/Fertility Clinic):
Both Partners:
- History:
- Duration of infertility, frequency of intercourse, previous pregnancies/children
- Menstrual history (regularity suggests ovulation)
- Medical history: Pelvic surgery, PID, STIs, testicular trauma, chronic illness
- Medications, smoking, alcohol, recreational drugs
- Occupational exposures (heat, chemicals)
- Lifestyle advice: BMI 19-25 optimal, smoking cessation, reduce alcohol (\<14 units/week), folic acid 400mcg daily (female), avoid saunas/hot baths (male)
Female Investigations:
- 1. Confirm ovulation:
- Mid-luteal progesterone (Day 21 of 28-day cycle): >30 nmol/L confirms ovulation
- Time 7 days before expected next period (not always day 21 if irregular cycles)
- If <30 nmol/L → repeat, consider ovulatory dysfunction
- 2. Assess ovarian reserve (especially if age ≥35):
- AMH (Anti-Müllerian Hormone): Can be done any day of cycle
- >15 pmol/L = normal reserve
- 5-15 pmol/L = low reserve
- <5 pmol/L = very low reserve
- Day 2-5 FSH: <10 IU/L normal, >15 IU/L suggests low reserve
- 3. Screen for ovulatory disorders:
- Day 2-5: FSH, LH, oestradiol, prolactin, TSH, testosterone (if PCOS suspected)
- 4. Assess tubal patency:
- Hysterosalpingogram (HSG): X-ray with contrast dye to check tubal patency
- Outpatient procedure, done days 5-12 of cycle (before ovulation)
- Shows tubal blockage, uterine abnormalities, filling defects
- HyCoSy (Hysterosalpingo-contrast sonography): Ultrasound alternative to HSG
- Laparoscopy + dye test: Gold standard (also treats endometriosis/adhesions), but invasive
- 5. Infection screening:
- Chlamydia screen (endocervical swab or urine NAAT)
- Rubella immunity (vaccinate if non-immune, avoid pregnancy 1 month post-vaccine)
Male Investigations:
- Semen analysis (ESSENTIAL first test):
- 2-5 days abstinence before sample
- Assess: Volume, concentration, motility, morphology
- WHO 2010 reference values (lower limits of normal):
- Volume ≥1.5 mL
- Concentration ≥15 million/mL
- Total count ≥39 million
- Progressive motility ≥32%
- Normal forms ≥4%
- If abnormal: Repeat after 3 months (spermatogenesis cycle = 3 months)
- If azoospermia:
- FSH, LH, testosterone
- Genetic testing (karyotype for Klinefelter 47,XXY, cystic fibrosis gene if CBAVD)
- Testicular biopsy (distinguish obstructive vs non-obstructive)
Management of Infertility:
General Measures (All Couples):
- Lifestyle optimization:
- Achieve healthy BMI (19-25)
- Stop smoking (both partners)
- Reduce alcohol (\<14 units/week women, <21 units/week men)
- Folic acid 400mcg daily (female, preconception + first 12 weeks pregnancy)
- Regular intercourse (every 2-3 days) throughout cycle (no need to time exactly)
- Address reversible factors: Treat infections, optimize medications, avoid occupational exposures
Specific Treatments:
1. Ovulatory Disorders (Female):
- PCOS: Weight loss, clomifene citrate, letrozole, metformin, ovarian drilling
- Hyperprolactinaemia: Dopamine agonists (cabergoline)
- Hypothalamic amenorrhoea: Weight gain, reduce exercise, pulsatile GnRH or gonadotropins
2. Tubal Factor (Female):
- Mild tubal damage/adhesions: Laparoscopic adhesiolysis, tubal surgery
- Severe tubal damage/blockage: IVF (bypasses tubes)
- Hydrosalpinx: Salpingectomy before IVF (toxic fluid reduces IVF success)
3. Male Factor:
- Mild oligozoospermia:
- Lifestyle: Stop smoking, reduce alcohol, lose weight, avoid heat
- Treat varicocele if present (surgical ligation)
- Intrauterine insemination (IUI) with prepared sperm
- IVF with ICSI if IUI fails
- Severe oligozoospermia/azoospermia:
- IVF with ICSI (intracytoplasmic sperm injection): Single sperm injected directly into egg
- If obstructive azoospermia: Surgical sperm retrieval (PESA, TESA) + ICSI
- If non-obstructive azoospermia: Donor sperm
4. Unexplained Infertility:
- Expectant management: Continue trying naturally (up to 2 years) - many conceive spontaneously
- Ovulation induction + IUI: Modest improvement (if female age <35)
- IVF: Higher success rates (especially if age >35 or trying >2 years)
Assisted Reproduction Techniques (ART):
A) Intrauterine Insemination (IUI):
- Indications: Mild male factor, unexplained infertility, same-sex couples, single women (donor sperm)
- Procedure:
- Sperm sample prepared (washed, concentrated)
- Inseminated into uterus around ovulation (natural or stimulated cycle with clomifene/FSH)
- Success: 10-15% per cycle (cumulative 30-40% after 6 cycles)
- Max 6 cycles - if unsuccessful, move to IVF
B) In Vitro Fertilisation (IVF):
- Indications: Tubal factor, endometriosis, failed IUI, severe male factor (with ICSI), unexplained infertility
- Procedure:
- Ovarian stimulation: Daily FSH injections (10-14 days) to produce multiple eggs
- Egg retrieval: Transvaginal ultrasound-guided aspiration under sedation
- Fertilisation: Eggs + sperm mixed in lab (or ICSI if severe male factor)
- Embryo culture: 3-5 days (blastocyst stage)
- Embryo transfer: 1-2 embryos transferred to uterus via catheter
- Luteal support: Progesterone pessaries
- Pregnancy test: 2 weeks after transfer
- Success rates (per cycle):
- Age <35: 30-35%
- Age 35-37: 25-30%
- Age 38-40: 15-20%
- Age >40: \<10%
- Risks: Ovarian hyperstimulation syndrome (OHSS), multiple pregnancy (twins 10-15%), ectopic pregnancy
C) ICSI (Intracytoplasmic Sperm Injection):
- Single sperm injected directly into egg (part of IVF cycle)
- Indications: Severe oligozoospermia, previous IVF fertilisation failure, obstructive azoospermia
NHS Funding for IVF (England - 2024):
- Eligibility (NICE guidelines):
- Female aged <40 (some CCGs up to 42)
- Tried to conceive for 2 years (or 6 cycles IUI if donor sperm/same-sex couple)
- No previous children (either partner) - varies by CCG
- NHS offers: 1 full cycle (some CCGs offer 3 cycles if <40)
- Private IVF: £5,000-8,000 per cycle
This couple's management plan:
- Diagnosis: Male factor infertility (oligozoospermia 8-10 million/mL)
- Male partner (Mark):
- Lifestyle: Reduce alcohol (\<21 units/week), avoid hot baths/saunas, lose weight if overweight
- Scrotal ultrasound to exclude varicocele
- Repeat semen analysis in 3 months
- Fertility treatment options:
- Option 1: IUI (3-6 cycles): Prepared sperm inseminated at ovulation - success 10-15% per cycle
- Option 2: IVF with ICSI: If IUI fails or couple prefer - success 30% per cycle (Claire aged 33)
- Referral to fertility clinic for specialist assessment and treatment planning
- Female partner (Claire): Continue folic acid 400mcg daily, optimize health
- Prognosis: Good - Claire is young (33) with normal ovarian reserve and patent tubes. IVF with ICSI has 30-35% success per cycle.